Composite

Part:BBa_K1615078

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-28)

TVEL5 fused to Linker-CBDcipA-linker in RFC25

TVEL Laccase (BBa_K1615067) fused to the N terminal of CBDcipA with N and C terminal linkers (BBa_K1615111). If folded properly, this allows laccase to catalyse the reaction of O2 to 2H2O while immobilised on cellulose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
    Illegal BglII site found at 773
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 418


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Categories
Parameters
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